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The specialized transduction phage stoke was pre-incubated at 37°C while MAP cultures were being prepared for transduction as follows: a 10 ml culture of MAP A1-157 strain was grown to an OD 600 = 0.6-0.8 at 37°C, 140 rpm, the cultures were then subcultured at a 1:10 ratio into 7H9 media
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