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Data acquisition for ABPP Peptides were analysed by LC–MS/MS using an Evosep One (Evosep) coupled with a timsTOF HT (Bruker) equipped with either an 8 cm × 150 µm, 1.5 µm analytical column (Evosep, 60SPD, ABPP samples) or a 15 cm × 150 µm, 1.9 µm analytical column (Evosep, 30SPD, SK-MEL-103 total proteome samples)
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