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BS 3 crosslinking assay Purified protein samples were buffer exchanged into a crosslinking-compatible buffer (20 mM HEPES, pH 7.5, 150 mM NaCl) using 30 K Amicon Ultra centrifugal filters (Sigma Aldrich Inc., St

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Crystal structure of the antioxidant enzyme glutathione reductase inactivated by peroxynitrite