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Effects of glutathione reductase inhibition on cellular thiol redox state and related systems - PMC

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Quantitative evaluation of apoptosis was performed by flow cytometry after double staining using fluorescein FITC-conjugated Annexin V and 5 μg/ml Propidium iodide 10 min at room temperature, and analyzed by fluorescence-activated cell sorting (FACS) in the FL1 and FL2 channels to determine the percentage of dead cells