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As the most abundant subtype in the central nervous system, the stoichiometric ratio differences between (α 4 β 2 ) 2 β 2 and (α 4 β 2 ) 2 α 4 of α 4 β 2 nAChR determine receptor sensitivity and functional characteristics toward nicotine: The (α 4 β 2 ) 2 β 2 subtype exhibits high agonist affinity, whereas the (α 4 β 2 ) 2 α 4 subtype demonstrates 3–4-fold enhanced activation efficacy despite lower affinity, establishing a dual regulatory paradigm of “high-affinity” and “high-efficacy” (Gotti et al., 2009)

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In contrast, DHAR and GaILDH levels significantly rose on the seventh day