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The fluid complexity leads to several contamination issues, as the standard and efficient preparation methods, apart from the conventional methods (e.g., ultracentrifugation, ultrafiltration, gel filtration technique, etc.), remain underexplored, thus restricting the clinical development of exosome-based therapies (Yakubovich et al., 2022)

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In fact, protein crystallography was started by the pioneering work of John Desmond Bernal (1901–1971) and Dorothy Crowfoot Hodgkin (1910–1994), who reported the first X-ray photographs of crystalline pepsin in 1934 (Bernal and Crowfoot, 1934)