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Immunohistochemical analysis of focal demyelinated lesions utilized overall neuropathological changes (hematoxylin and eosin) along with myelin loss to determine the lesion core, while the lesion rim was defined as also encompassing neuroinflammatory markers (GFAP, IBA1)

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Metabolic Syndrome Models DIO mouse models: 5-Amino-1MQ reduces fat mass and improves insulin sensitivity without affecting food intake — distinct from appetite-suppression mechanisms