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RT-qPCR was used to measure gene expression levels of TNF-α, IL-6, IL-1β, Ccl2, Ccl4, TLR2, TLR4 in liver tissue, and ZO-1, occludin, claudin 1, and claudin 4 in colon tissue

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However, approval for NDA application was denied due to concerns by the FDA about this agent’s gastrointestinal and cardiovascular effects over long-term use (NicOx 2010)