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To control for the charge of ES2, ProteoJuice (Millipore Sigma, 71281) was used to deliver ES2 using the manufacturers protocol, except 50 µL of 2 mM ES2 was mixed with 25 µL of ProteoJuice

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Select Syringe Size x Gauge x Needle Length:1cc x 28g x 1/2"

Frontiers in Endocrinology