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The synaptoneurosome-containing pellet was then resuspended in the appropriate amount of lysis buffer containing 140 mM NaCl, 3 mM KCl, 10 mM Glucose, 2 mM MgSO 4, 2 mM CaCl 2 and 10 mM HEPES (pH 7.4) containing protease inhibitors and RNAsin

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