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Another study found that auditory stimulation significantly increased dopamine (DA) and 5-hydroxyindoleacetic acid (5-HIAA) concentrations in adult male Wistar rats, but had no effect on dihydroxyphenylacetic acid (DOPAC) or 5-HT levels ( 4.2.2 The glutamatergic system The glutaminergic system plays a crucial role in the PFC, hippocampus and amygdala, which play a central role in regulating stress-related emotions of sleep, anxiety and fear