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Sulfenylated cysteines are protected from further oxidation either by glutathionylation (S-glutathionylation) or by reduction, e.g., via the Trx1 system

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Here, we have utilized liver-specific overexpression of cation transport regulator homolog 1 (Chac1), a recently identified intracellular GSH-degrading enzyme, to induce hepatic GSH depletion during MASLD progression