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TB-500 Nasal Spray, 8mg (0.10ml per serving), For Research Only

Sample preparation for T cell proteomics A375 melanoma tumor cells (ATCC, CRL-1619) were cultured in complete DMEM (Gibco, 11965-092) supplemented with 10% (v/v) FBS (VWR, 97068-085) and 1% penicillin–streptomycin (VWR, 16777-164) at 37 °C under 5% CO 2

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For colony formation assays, cells (5 × 10 4 ) were suspended in 2 mL of 0.33% noble agar (Difco) and laid over 6-well culture plates previously coated with 2 mL of 0.5% noble agar