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The amount of formazan that is generated corresponds to the number of live cells [49] In brief, SH-SY5Y cells were seeded at a density of 5 × 10 4 in a 96-well plate (6 wells/concentration group plus 1 control group) and, following various treatments, cells were incubated with 20 μL of MTT stock solution (5 mg/mL in PBS 1X) in 180 μL of medium in the dark
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Do not take this product if taking the following medications: 5-fluorouracil, ACE inhibitors, activated charcoal, aminoglycoside antibiotics, amiodarone, antacids, anticoagulants/antiplatelet drugs, antidiabetic drugs, antihypertensive drugs, atazanavir, bisphosphonates, blood thinners, calcium channel blockers, capecitabine, cephalexin, chloroquine, cisplatin, digoxin, fosphenytoin, integrase inhibitors, levodopa/carbidopa, methotrexate, nitroglycerin, phenobarbital, penicillamine, phenytoin, potassium-sparing diuretics, primidone, pyrimethamine, quinolone antibiotics, ritonavir, skeletal muscle relaxants, sulfonylureas, and/or tetracycline antibiotics