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In affinity chromatography, selectivity towards a specific target protein is introduced through the chemical functionalization of the solid support with desired affinity ligands, which can be divided into three main categories: biological, structural and synthetic (Fig

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Furthermore, as new insights are made into the transcriptional regulation of appetite-regulating neuropeptides as well as the genes involved in cellular metabolism, new drugs targeting these pathways may have potential as anti-obesity pharmacotherapeutics