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Protein precipitates were sonicated (30 s pulse, bath-type sonicator) for 10 min, vortexed, resuspended in 450 μl of 0.1 M PBS, vortexed, centrifuged (10,000 xg for 10 min at 4°C) and the supernatant aliquoted (150 μl/well) in triplicate into a black 96-well microplate (Thermo Scientific™)
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cruzi , and its inhibition increased the carbonylated protein content, resulting from formation of covalent, non-reversible lipid aldehyde adducts on side chain of cysteine, histidine, and lysine residues