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DNA hypermethylation caused by loss of TET activity increases nucleosome occupancy, and subsequently, loss of CTCF binding and a block in the downstream recruitment of the cohesin complex, reducing the formation of CTCF/cohesion-mediated chromatin loops thereby downregulating the expression of neighboring genes (Wiehle et al., 2019

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The first clinical trials tested CARs that had a binding domain composed of native CD4 that bound to the envelope glycoprotein gp120 expressed by HIV-infected cells, with a single T cell receptor (TCR) signaling domain composed of the CD3ζ chain