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Afterwards, the skin was rinsed with PBS and each of the skin explants was divided into thirds for histological examination (4% PFA fixation at 4 °C), protein analysis by means of Western blot (snap freezing with liquid nitrogen), and qPCR (overnight in RNAlater (Thermo Fisher Scientific), then at − 80 °C)

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Neckline: Off-the-Shoulder & Sweetheart Neckline

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